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I just recently went from an XSi to a 5D and I would say that it's going to take some getting used to the controls. At first I was like "WTF is this??" but then it became second nature. Of course the main reasons for my upgrade was the size (fits better), build quality, better performance at higher ISO, and of course full frame (although this doesn't matter to me too much). If you are only upgrading because of IQ then I would say it's not worth it. Unless it's a fairly big upgrade, you won't notice that much difference in IQ. The camera does make a difference in pictures though (not just lenses). With cameras like 50D you can do microadjustments for the lenses and some other useful features.
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